ABSTRACT
BACKGROUND: This study was to investigate the effect of biomechanical stimulation on osteoblast differentiation of human periosteal-derived stem cell using the newly developed bioreactor. METHODS: Human periosteal-derived stem cells were harvested from the mandible during the extraction of an impacted third molar. Using the new bioreactor, 4% cyclic equibiaxial tension force (0.5 Hz) was applied for 2 and 8 h on the stem cells and cultured for 3, 7, and 14 days on the osteogenic medium. Biochemical changes of the osteoblasts after the biomechanical stimulation were investigated. No treatment group was referred to as control group. RESULTS: Alkaline phosphatase (ALP) activity and ALP messenger RNA (mRNA) expression level were higher in the strain group than those in the control group. The osteocalcin and osteonectin mRNA expressions were higher in the strain group compared to those in the control group on days 7 and 14. The vascular endothelial growth factor (VEGF) mRNA expression was higher in the strain group in comparison to that in the control group. Concentration of alizarin red S corresponding to calcium content was higher in the strain group than in the control group. CONCLUSIONS: The study suggests that cyclic tension force could influence the osteoblast differentiation of periosteal-derived stem cells under optimal stimulation condition and the force could be applicable for tissue engineering.
Subject(s)
Humans , Alkaline Phosphatase , Bioreactors , Calcium , Jaw , Mandible , Molar, Third , Osteoblasts , Osteocalcin , Osteonectin , RNA, Messenger , Stem Cells , Tissue Engineering , Vascular Endothelial Growth Factor AABSTRACT
Subject(s)
Humans , Carcinoma, Squamous Cell , Diagnosis , Follow-Up Studies , Leukoplakia , Mouth , Neoplasm Recurrence, Local , Neoplasms, Second Primary , Prognosis , Recurrence , Tongue Neoplasms , TongueABSTRACT
Subject(s)
Female , Humans , Biopsy , Carcinoma, Squamous Cell , Cheek , Forearm , Free Tissue Flaps , Neck Dissection , Radial Artery , Radiation Oncology , Skin , Thigh , Tissue Donors , TransplantsABSTRACT
INTRODUCTION: Fibrous-osseous lesions of the jaws are difficult to diagnose precisely until excised biopsy results are found, so they might be confused with malignant lesions. This clinical study focused on the diagnostic aids of lesions that demonstrate different clinical, radiologic, and histological findings. MATERIALS AND METHODS: A total of 16 patients with benign fibrous-osseous lesions on the jaws (6 fibrous dysplasias, 6 ossifying fibromas, 3 cemental dysplasias, and one osteoblastoma) were reviewed. Nine patients with malignant fibrous-osseous lesions (8 osteosarcomas and one Ewing's sarcoma) were also retrospectively reviewed. RESULTS: Osteosarcoma patients complained of facial swelling and tooth mobility. The radiographic findings showed the irregular resorption of cortical bone and periosteal reactions. Histological features included cellular pleomorphism and atypical mitosis. An Ewing's sarcoma patient complained of tooth mobility and facial swelling. Onion-skin appearance and irregular expansile marginal bony radiolucency were seen in the radiography. Fibrous dysplasia patients complained of facial swelling and asymmetry. The radiographic features were mostly ground-glass radiopacity. Histological findings showed a bony trabeculae pattern surrounded by fibrous ground substances. Ossifying fibroma patients complained of buccal swelling and jaw pains, showing expanded cortical radiolucent lesions with a radiopaque margin. Histological findings were revealed as cellular fibrous stroma with immature woven bones. In cemental dysplasia, most of their lesions were found in a routine dental exam. Well-circumscribed radiopaque lesions were observed in the radiography, and cementum-like ossicles with fibrous stroma were seen in the microscopy. An osteoblastoma patient complained of jaw pain and facial swelling. Radiographic findings were mottled, dense radiopacity with osteolytic margin. Trabeculae of the osteoid with a vascular network and numerous osteoblasts with woven bone were predominantly found in the microscopy. CONCLUSION: Our study showed similar results as other studies. We suggest the clinical parameters of diagnosis and treatment for malignant and benign fibrous-osseous lesions of the jaws.
Subject(s)
Humans , Biopsy , Fibroma, Ossifying , Jaw , Microscopy , Mitosis , Osteoblastoma , Osteoblasts , Osteosarcoma , Retrospective Studies , Sarcoma, Ewing , Tooth MobilityABSTRACT
Subject(s)
Humans , Male , Middle Aged , Facial Injuries , Foreign Bodies , Forensic Ballistics , Lead Poisoning , Shock , Temporomandibular Joint , Wounds, GunshotABSTRACT
PURPOSE: The present study was performed to compare the treatment outcomes of non-surgical periodontal treatment according to the distribution of attachment loss of a given patient. METHODS: Forty-five patients with moderate to severe periodontitis were divided in two subgroups; Group I patients with teeth manifesting attachment loss of > or =6 mm at one or more sites on the buccal/labial aspect while maintaining an attachment level or =6 mm at more than one site on the lingual/palatal aspect while maintaining an attachment level or =6 mm at buccal/labial surfaces responded better to the nonsurgical periodontal therapy than those demonstrating comparable attachment loss at lingual/palatal surfaces.
Subject(s)
Humans , Mastication , Periodontal Pocket , Periodontitis , Prognosis , Tooth , Tooth MobilityABSTRACT
Subject(s)
Animals , Rabbits , Calcium , Femur , Nitrogen Mustard Compounds , OsseointegrationABSTRACT
Subject(s)
Humans , Adenoma, Pleomorphic , Congenital Abnormalities , Cosmetics , Depression , Facial Nerve Injuries , Incidence , Sweating, GustatoryABSTRACT
Subject(s)
Bone Density , Cone-Beam Computed Tomography , Dental Implants , Follow-Up Studies , Maxilla , OsseointegrationABSTRACT
PURPOSE: The purpose of this study is to investigate the clinical and histological features of odontogenic keratocyst PATIENTS AND METHODS: A retrosective review of 100 patients who were diagnosed as odontogenic keratocyst by hitological findings during the period of January 2000 and December 2005 in the Dept. of Oral and Maxillofacial surgery Pusan National University was consecuted. For each patient, age, sex, location of lesion, initial diagnosis by radiographic features, treatment procedure, hitologic findings and recurrance rate were evaluated. RESULTS: In this study, OKC has male prevalance to female by 1.38:1, and most likely occurs during third decade. The most common site of lesion was mandibular ramus region(34.6%) and the most common symptom was swelling(50%). The most common initial diagnosis by radiographic findings was OKC and cyst enucleation was the most common treatment method. The recurrance rate was 28% and existence of daugther cyst is thought to be most convincing factor for prediction of recurrence. CONCLUSION: In this study, total recurrence rate was 28% and existence of daugther cyst is thought to be most convincing factor for prediction of recurrence. But, since 97% of patients were treated by enucleation and adjuntive excision, further styudy is need about concordance of recurrence rate with surgical method.